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    [机翻] 中国东部和美国东部空气污染的化学特征
    摘要 : Satellite data (MODIS, GOME, and MOPITT) together with a chemical transport global model of the atmosphere (MOZART-2) are used to characterize air pollution in Eastern China and the Eastern US to assess the differences between the... 展开

    摘要 : To accurately determine the space use of animals, we need to follow animal movements over prolonged periods, which is especially challenging for the critically endangered Grauer's gorillas (Gorilla beringei graueri) in eastern Dem... 展开

    摘要 : Land-use changes or plant species have a profound impact on soil chemical properties, but under their combined action, little is known about the changes in soil fungal communities and functional guilds. We investigated the effects... 展开

    摘要 : 乾燥地帯に起原した乳文化が湿潤地帯に伝播した際, どのように文化変遷するかを検討するために, インド東 北部ァルナチャル'プラデーシュ州ウェスト、カメン県 ディラン、サークルの湿潤地帯でチべット系牧畜民を対 象に調査をおこなった。インド北... 展开

    摘要 : Statistical models for the seasonal prediction of hurricane strikes on the Eastern Seaboard of the United States (ESUS) are developed with the application of the statistical software (SAS) generalized linear model (GENMOD) to perf... 展开

    摘要 : Introduction: Recurrence patterns after curative intent gastrectomy for T1-2N0 gastric adenocarcinoma are poorly defined. We sought to assess timing, patterns, and risk factors for recurrence in patients treated at two high-volume... 展开

    摘要 : Soil hydrological properties and aggregate stability are strongly impacted by erosion and management practices. However, magnitudes of the erosion-induced changes in topsoil depth and the attendant alterations in soil properties a... 展开

    摘要 : Habitat loss threatens large mammals worldwide, and their survival will depend on habitat in human-dominated landscapes. Conservation planners thus face the challenge to identify areas of least conflict with land use, yet broadsca... 展开

    摘要 : Agriculture is expanding and intensifying in many areas of the world, but abandoned agriculture is also becoming more widespread. Unfortunately, data and methods to monitor abandoned agriculture accurately over large areas are lac... 展开

    摘要 : Maize lethal necrosis (MLN) disease is a major constraint on maize production in Eastern Africa since its first report in 2012 in Kenya. The main causative agent is maize chlorotic mottle virus (MCMV) which co-infects maize with o... 展开
    关键词 : Maize lethal necrosis (MLN) disease is a major constraint on maize production in Eastern Africa since its first report in 2012 in Kenya. The main causative agent is maize chlorotic mottle virus (MCMV) which co-infects maize with other viruses from the family Potyviridae. In AfricaSugarcane mosaic virus (SCMV) is the most common potyvirus that co-infects maize synergistically with MCMV. MCMV can be transmitted by insect vectorsmechanically and via contaminated seeds. Monitoring of MCMV is important in farmers&rsquo   fieldsseed fieldsseed-lots and in grain. Robust diagnostic tools are essential in epidemiological studiesgermplasm screening and exchange of disease-free materials across the regions and globally. Thereforea sensitivereliable and affordable diagnostic tool for MCMV is necessary in the laboratory and also in the field. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay with two-end point analysis was developed to detect MCMV in active vegetative stages and in seed. Six sets of specific primers were designed and evaluated. Amplification was detected in 60 min using the SYBR green colour change and in 10 to 20 min for real-time amplification in the Genie II platform. The assay discriminated the common viruses infecting maize in Eastern Africa. The assay showed excellent specificity to MCMV. The simplicityrapidityand inexpensiveness of this technique make it a suitable choice for large-scale sample processingespecially by laboratories with limited resources and for field analysis performed by regulatory agencies in the region. Key word: Maize lethal necrosismaize chlorotic mottle virusdiagnosticsloop mediated isothermal amplification.  

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